Production of Goat Pox Virus Vaccine from a Live Attenuated Goat Pox Virus Strain
نویسندگان
چکیده
Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days. Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers. Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation. TCID50 calculated for the pool virus was 10 / ml. Vaccine was found free from any bacterial and mycoplasma contamination. All mice and guinea pigs were found healthy after 21days post vaccinal administration. Vaccinated goats challenged after 14 days with 1 ml of virulent goat pox virus (TCID50 10/ml) did not show any unwanted reaction while controls challenged with 1 ml of virulent goat pox virus (TCID50 10 /ml) showed pox lesions and rise in body temperature. The main objective of this study was to produce and study the efficacy of this vaccine.
منابع مشابه
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